cancerspЮжно-Российский онкологический журнал/ South Russian Journal of CancerSouth Russian Journal of Cancer2686-9039АНО "Перспективы онкологии"10.37748/2686-9039-2024-5-3-8AVXOUIcancersp-313Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESМетаболомный профиль злокачественных опухолей яичникаMetabolomic profile of malignant ovarian tumorsФилипповФ. Е.FilippovF. E.

Филиппов Федор Евгеньевич – врач онколог отделения онкогинекологии, ГБУЗ «Клинический онкологический диспансер № 1» Министерства здравоохранения Краснодарского края, г. Краснодар, Российская Федерация

Fedor E. Filippov – oncologist of the Department of Oncogynecology, Clinical Oncology Dispensary No. 1, Krasnodar, Russian Federation

https://orcid.org/0000-0002-8942-3733КутилинД. С.KutilinD. S.

Кутилин Денис Сергеевич – к.б.н., ведущий научный сотрудник лаборатории молекулярной онкологии, ФГБУ «Национальный медицинский исследовательский центр онкологии» Министерства здравоохранения Российской Федерации, г. Ростов-на-Дону, Российская Федерация

ORCID: https://orcid.org/0000-0002-8942-3733, SPIN: 8382-4460, AuthorID: 794680, Scopus Author ID: 55328886800

Denis S. Kutilin – Cand. Sci. (Biol.), Leading Researcher, Laboratory of Molecular Oncology, National Medical Research Centre for Oncology, Rostov-on-Don, Russian Federation

ORCID: https://orcid.org/0000-0002-8942-3733, SPIN: 8382-4460, AuthorID: 794680, Scopus Author ID: 55328886800

k.denees@yandex.ruhttps://orcid.org/0000-0002-9471-3903МаксимовА. Ю.MaksimovA. Yu.

Максимов Алексей Юрьевич – д.м.н., профессор, заместитель генерального директора, ФГБУ «Национальный медицинский исследовательский центр онкологии» Министерства здравоохранения Российской Федерации, г. Ростов-на-Дону, Российская Федерация

ORCID: https://orcid.org/0000-0002-9471-3903, SPIN: 7322-5589, AuthorID: 710705, Scopus Author ID: 56579049500

Aleksey Yu. Maksimov – Dr. Sci. (Med.), Professor, Deputy Director General, National Medical Research Centre for Oncology, Rostov-on-Don, Russian Federation

ORCID: https://orcid.org/0000-0002-9471-3903, SPIN: 7322-5589, AuthorID: 710705, Scopus Author ID: 56579049500

ПорхановаН. В.PorkhanovaN. V.

Порханова Наталья Владимировна – д.м.н., доцент кафедры онкологии, врач онколог, ГБУЗ «Клинический онкологический диспансер № 1» Министерства здравоохранения Краснодарского края, г. Краснодар, Российская Федерация

SPIN: 2611-4840, AuthorID: 589928

Natalya V. Porhanova – Dr. Sci. (Med.), Associate Professor of the Department of Oncology, oncologist, Clinical Oncology Dispensary No. 1, Russian Federation, Krasnodar, Russian Federation

SPIN: 2611-4840, AuthorID: 589928

ГБУЗ «Клинический онкологический диспансер № 1» Министерства здравоохранения Краснодарского краяРоссияClinical Oncology Dispensary No. 1Russian FederationФГБУ «Национальный медицинский исследовательский центр онкологии» Министерства здравоохранения Российской ФедерацииРоссияNational Medical Research Centre for OncologyRussian Federation2024190920245391101Copyright © Филиппов Ф.Е., Кутилин Д.С., Максимов А.Ю., Порханова Н.В., 20242024Филиппов Ф.Е., Кутилин Д.С., Максимов А.Ю., Порханова Н.В.Filippov F.E., Kutilin D.S., Maksimov A.Y., Porkhanova N.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.cancersp.com/jour/article/view/313Цель исследования

Цель исследования. Изучение метаболомного профиля в тканях у больных серозной аденокарциномой яичников.

Материалы и методы

Материалы и методы. В исследование было включено 100 пациенток с диагнозом серозная аденокарцинома яичников. Хроматографическое разделение метаболитов проводили на хроматографе Vanquish Flex UHPLC System, который был сопряжен с масс-спектрометром Orbitrap Exploris 480. Оценку различий проводили с использованием критерия Манна‑Уитни с поправкой Бонферрони.

Результаты

Результаты. В опухолевой ткани яичника 20 соединений имели аномальную концентрацию по сравнению с нормальной тканью: обнаружено увеличение содержания кинуренина, фенилаланил-валина, лизофосфатидилхолина (18:3), лизофосфатидилхолина (18:2), аланил-лейцина, L-фенилаланина, фосфатидилинозитола (34:1), 5-метокситриптофана, лизофосфатидилхолина (14:0), индолакриловой кислоты и снижение содержания миристиновой кислоты, деканоилкарнитина, аспартил-глицина, малонилкарнитина, 3-метилксантина, 3-оксододекановой кислоты, 2-гидроксимиристиновой кислоты, N-ацетилпролина, L-октаноилкарнитина и каприлоилглицина.

Заключение

Заключение. В опухолевой ткани яичника обнаружен значительный метаболомный дисбаланс, выраженный в аномальных концентрациях жирных кислот и их производных, ацилкарнитинов, аминокислот и их производных, фосфолипидов и производных азотистых оснований. Концентрации этих 20 метаболитов в тканях могут служить диагностическими маркерами рака яичников. Таким образом, метаболомное профилирование тканей позволило как выявить потенциальные маркеры заболевания, так и лучше понять молекулярные механизмы изменений, лежащих в основе развития данного заболевания.

Purpose of the study

Purpose of the study. Investigate the metabolomic profile in tissues of patients with serous ovarian adenocarcinoma.

Materials and methods

Materials and methods. The study included 100 patients with serous ovarian adenocarcinoma. Chromatographic separation of metabolites was performed on a Vanquish Flex UHPLC System chromatograph, which was coupled with an Orbitrap Exploris 480 mass spectrometer. Differences were assessed using the Mann-Whitney test with Bonferroni correction.

Results

Results. In ovarian tumor tissue, 20 compounds had abnormal concentrations compared to normal tissue: increased levels of kynurenine, phenylalanylvaline, lysophosphatidylcholine (18:3), lysophosphatidylcholine (18:2), alanylleucine, L-phenylalanine, phosphatidylinositol (34:1), 5-methoxytryptophan, lysophosphatidylcholine (14:0), indoleacrylic acid and decreased levels of myristic acid, decanoylcarnitine, aspartylglycine, malonylcarnitine, 3-methylxanthine, 3-oxododecanoic acid, 2-hydroxymyristic acid, N-acetylproline, L-octanoylcarnitine and capryloylglycine.

Conclusion

Conclusion. A significant metabolic imbalance was found in ovarian tumor tissue, expressed in abnormal concentrations of fatty acids and their derivatives, acylcarnitines, amino acids and their derivatives, phospholipids and nitrogenous base derivatives. The concentrations of these 20 metabolites in tissues can serve as diagnostic markers of ovarian cancer. Thus, metabolomic tissue profiling allowed both to identify potential markers of the disease and to better understand the molecular mechanisms of changes underlying the development of this disease.

метаболитыультра-высокоэффективная жидкостная хроматография и масс-спектрометриясерозная аденокарцинома яичникабиомаркеры.metabolitesultra-high performance liquid chromatography and mass spectrometryovarian serous adenocarcinomabiomarkersфинансирование данной работы не проводилось. Работа выполнена с использованием научного оборудования ЦКП ФГБУ «Национальный медицинский исследовательский центр онкологии» Министерства здравоохранения Российской Федерации: https://ckp-rf.ru/catalog/ckp/3554742/this work was not funded. The work was performed with scientific equipment provided by the Central Research Institute of the National Medical Research Center for Oncology: https://ckp-rf.ru/catalog/ckp/3554742/ReferencesReid BM, Permuth JB, Sellers TA. Epidemiology of ovarian cancer: a review. Cancer Biol Med. 2017 Feb;14(1):9–32. https://doi.org/10.20892/j.issn.2095-3941.2016.0084Reid BM, Permuth JB, Sellers TA. Epidemiology of ovarian cancer: a review. 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The authors declare that there are no conflicts of interest present.