cancerspЮжно-Российский онкологический журнал/ South Russian Journal of CancerSouth Russian Journal of Cancer2686-9039АНО "Перспективы онкологии"10.37748/2687-0533-2020-1-3-4cancersp-72Research ArticleОБЗОРЫREVIEWSПервичные культуры опухолевых клеток: современные методы получения и поддержания in vitroPrimary tumor cell cultures: сurrent methods of obtaining and subcultivationhttps://orcid.org/0000-0002-7902-7278МежевоваИ. В.MezhevovaI. V.

Межевова Ирина Валентиновна – младший научный сотрудник Лаборатории клеточных технологий. SPIN: 3367-1741, AuthorID: 1011695, ResearcherID: AAI-1860-2019

344037, г. Ростов-на-Дону, ул. 14-я линия, д. 63

Irina V. Mezhevova – junior researcher, laboratory of cell technologies. AuthorID: 1011695, ResearcherID: AAI-1860-2019

63 14 line str., Rostov-on-Don 344037

mezhevova88@gmail.comhttps://orcid.org/0000-0002-6035-1756СитковскаяА. О.SitkovskayaA. O.

Ситковская Анастасия Олеговна – ВРИО заведующей Лаборатории клеточных технологий. SPIN: 1659-6976, AuthorID: 791081, Scopus Author ID: 56381527400, ResearcherID: E-7496-2018

344037, г. Ростов-на-Дону, ул. 14-я линия, д. 63

Anastasiya O. Sitkovskaya — Head of the Laboratory of Cell Technologies. AuthorID: 791081, Scopus Author ID: 56381527400, ResearcherID: E-7496-2018

63 14 line str., Rostov-on-Don 344037

https://orcid.org/0000-0003-3061-6108КитО. И.KitO. I.

Кит Олег Иванович – член-корр. РАН, д.м.н., профессор, генеральный директор. SPIN: 1728-0329, AuthorID: 343182, Scopus Author ID: 55994103100, ResearcherID: U-2241-2017

344037, г. Ростов-на-Дону, ул. 14-я линия, д. 63

Oleg I. Kit – member Russian Academy of Sciences, Dr. Sci. (Med.), professor, general director. SPIN: 1728-0329, AuthorID: 343182, Scopus Author ID: 55994103100, ResearcherID: U-2241-2017

63 14 line str., Rostov-on-Don 344037

ФГБУ «НМИЦ онкологии» Минздрава РоссииРоссияNational Medical Research Centre for Oncology of the Ministry of Health of RussiaRussian Federation202027082020133649Copyright © Межевова И.В., Ситковская А.О., Кит О.И., 20202020Межевова И.В., Ситковская А.О., Кит О.И.Mezhevova I.V., Sitkovskaya A.O., Kit O.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.cancersp.com/jour/article/view/72

В течение последних десятилетий перевиваемые клеточные линии являлись доступной моделью для изучения биологии и влияния химиотерапевтических препаратов на опухоли. Однако, многочисленные исследования показали, что данные клеточные линии недостаточно гетерогенны и не могут отражать лекарственную резистентность опухолей, возникающую у некоторых пациентов. Культуры первичных клеточных линий, выделенные из солидных опухолей, получили значительное распространение для определения химиочувствительности опухолей к препаратам, применяемым в химиотерапии. В данном обзоре рассматриваются основные методы получения и культивирования первичных клеточных линий. Дается краткая характеристика методикам дезагрегации опухолевого материала при помощи ферментативной, химической и механической диссоциации. Рассмотрены различные системы культивирования первичных клеточных культур. Выбор подходящего метода диссоциации и культивирования имеет важное значение для сохранения преимуществ первичной культуры в доклинических исследованиях.

Over the past decades, transplantable cell lines have been an affordable model for studying the biology and effect of chemotherapeutic drugs on tumors. However, numerous studies have shown that these cell lines are not heterogeneous enough and cannot reflect the drug resistance of tumors that occurs in some patients. Primary cell line cultures isolated from solid tumors have become widespread in personalized cancer therapy. This review discusses the basic methods for the preparation and cultivation of primary cell lines. A brief description is given of the methods for the disaggregation of tumor material using enzymatic, chemical and mechanical dissociation. The systems of cultivation of primary cell cultures. The selection of an appropriate dissociation method and cultivation is important to preserve the benefits of primary culture in preclinical studies.

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The authors declare that there are no conflicts of interest present.