The effects of combined action of low-intensity electromagnetic radiation of the millimeter range and complexes of essential amino acids in tumor-bearing rats of old age

Purpose of the study. Amplifying the effects of low-intensity modulated electromagnetic radiation (EMR) of the extremely high frequency range (EHF) on the tumor and adaptational status of old tumor-bearing rats with the help of complexes of essential amino acids enriched withvitamins and microelements (AaVM).

Materials and methods. In experiments on 50 old white outbred male rats with transplantable sarcoma 45, the effects of EMR EHF and the combined effect of EMR EHF and AaVM were studied. For exposure to EHF EHF, the "Yav-1" medical device was used. The frequency modulation with 42.2 GHz as a center frequency was applayed. The duration of treatmaent was 4 weeks. We studied the dynamics of tumor size, adaptive status (according to hematological indicators of the character and tension of the general nonspecific adaptational reactions of the body), structural and functional changes in the tumor tissue, thymus and liver. In the statistical analysis of the results, student's t test and Wilcoxon-Mann-Whitney test were used.

Results. The anti-stress effect of EMR EHF on the organism of old tumor-bearing rats, accompanied by temporary inhibition of sarcoma 45 growth was shown. The combination of EMR EHF exsposure and AaVM (as an additional metabolic factor) leaded to a persistent pronounced antitumor effect in 60% of animals. Inhibition of tumor growth in 4 times or more, as well as individual cases of its partial regression were observed. At the same time, a higher adaptational status including more numerous signs of activation of the thymus and liver than in the cases of using only EHF EMR were noted.

Conclusion. The results indicate the promise of a combination of activation electromagnetic therapy and application of complexes of essential amino acids enriched with vitamins and microelements in the development of effective methods of accompanying and supportive treatment of cancer patients.

1. Рекомендации по поддерживающей и сопроводительной терапии RUSSCO. 2019. Доступно по: https://rosoncoweb.ru/standarts/suptherapy/2019/

2. Чиссов В.И., Кабисов Р.К., Плетнев С.Д., Бецкий О.В., Файкин В.В. Способ лечения онкологических больных с помощью электромагнитного излучения мм-диапазона. Патент на изобретение RU 98 102 380 А, 27.01.2000. Заявка: 98102380/14 от 1998.01.23

3. Колмацуй Н.Б., Левицкий Е. Ф., Голосова О. Е., Пыжова И.Б. КВЧ-терапия в профилактике осложнений специфической антибластомной терапии у больных раком легкого III-IV стадии. Вопросы курортологии, физиотерапии и лечебной физической культуры. 2013;90(2):4-7.

4. Бецкий О.В., Лебедева Н.Н. Биологические эффекты низкоинтенсивных миллиметровых волн (обзор). Биомедицинская радиоэлектроника. 2015;(1):31-47.

5. Heyland DK, Weijs PJM, Coss-Bu JA, Taylor B, Kristof AS, O’Keefe GE, et al. Protein Delivery in the Intensive Care Unit: Optimal or Suboptimal? Nutr Clin Pract. 2017 Apr;32(1_sup-pl):58S-71S. https://doi.org/10.1177/0884533617691245

6. Костюченко Л.Н., Костюченко М.В., Кузьмина Т.Н., Лычкова А.Э. Стратегия сопроводительной нутриционно-метаболической терапии больных колоректальным раком. РМЖ. 2019;(6):37-44.

7. Garkavi LK, Kvakina EB, Shikhliarova AI, Kuz’menko TS, Barsukova LP, Mar’ianovskaia GI, et al. Magnetic fields, adaptation reaction and self-organization of live systems. Biofizika. 1996 Aug;41(4):898-905.

8. Zhukova GV, Shikhlyarova AI, Barteneva TA, Barsukova LP, Kostrovitskiy YV, et al. Some strategies of activation therapy using radiations of microwave ranges in experiments on tumor-bearing animals. Cardiometry. 2015 Nov 27;(7):47-57. https://doi.org/10.12710/cardiometry.2015.7.4757

9. Zhukova GV, Shikhliarova AI, Soldatov AV, Barteneva TA, Goroshinskaya IA, Petrosian VI, et al. Some approaches to the activation of antitumor resistance mechanisms and functional analogs in the categories of synergetics. BIOPHYSICS. 2016 Mar 1;61(2):303-315. https://doi.org/10.1134/S0006350916020251

10. Kit Kit OI, Shikhlyarova AI, Zhukova GV, Maryanovska-ya GY, Barsukova LP, et al. Activation therapy: theoretical and applied aspects. Cardiometry. 2015 Nov 27;(7):22-29. https://doi.org/10.12710/cardiometry.2015.7.2229

11. Vettore, L, Westbrook RL, Tennant DA. New aspects of amino acid metabolism in cancer. British Journal of Cancer. 2020 Jan;122(2):150-156. https://doi.org/10.1038/s41416-019-0620-5

12. Lieu, EL, Nguyen T, Rhyne S, Kim J. Amino acids in cancer. Exp Mol Med. 2020;52(1):15-30. https://doi.org/10.1038/s12276-020-0375-3

13. Al-Mahtab M, Akbar SMF, Khan MSI, Rahman S. Increased survival of patients with end-stage hepatocellular carcinoma due to intake of ONCOXIN®, a dietary supplement. Indian J Cancer. 2015 Sep;52(3):443-446. https://doi.org/10.4103/0019-509X.176699

14. Dzhugashvili M., Pokrovsky V.S., Snegovoy A.V. Novel approaches for the correction of micronutrient deficiency in patients with malignant tumors. Malignant Tumours 2016;2:55-65. https://doi.org/10.18027/2224-5057-2016-2-55-65

15. Покровский В.С., Лукашев Е.В., Чернов Н.Н., Трещалина Е.М. Экспериментальное изучение эффективности L-лизин-а-оксидазы Trichoderma CF. AureovirideRifai на моделях ксенографтов опухолей человека у бестимусных мышей и оценка синергизма с цисплатином или ингибиторами топоизомераз. Вопросы онкологии. 2017;63(3):497-505.

16. Коноплев В.П. Перевивные опухоли. В кн.: Модели и методы экспериментальной онкологии. М: Медгиз., 1960, 144-162 с.

17. Zhang C, He Y, Shen Y. L-Lysine protects against sepsis-induced chronic lung injury in male albino rats. Biomed Pharmacother. 2019 Sep;117:109043. https://doi.org/10.1016/j.biopha.2019.109043

18. Selye H. Thymus and adrenals in the response of the organisms to injuries and intoxication. Brit. J. Exp. Path. 1936;(17): 234-248.

19. Zhukova GV, Shikhlyarova AI, Barteneva TA, Bragina MI, Gudtskova TN, Petrosyan VM, et al. The pattern of the immune processes during activation therapy with the use of microwave electromagnetic radiation. Cardiometry. 2017;(11):35-45. https://doi.org/10.12710/cardiometry.2017.11.3545

20. Bishop GA. B cell-T cell interaction: antigen bridge to antigen presentation. Nat Rev Immunol. 2016;16(8):467. https://doi.org/10.1038/nri.2016.82